anti human ulbp3 antibodies (R&D Systems)
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Anti Human Ulbp3 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+ulbp3/Human+ULBP-3+Antibody/pm41529511-90-13-19
Average 94 stars, based on 15 article reviews
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Incubation:Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), anti- ULBP1 (1:50; MAB1380, R&D Systems), Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), antiULBP1 (1:50; MAB1380, R&D Systems), Binding Assay:Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), anti- ULBP1 (1:50; MAB1380, R&D Systems), Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), antiULBP1 (1:50; MAB1380, R&D Systems), Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer. Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), Flow Cytometry:Article Title: Reovirus infection of tumor cells reduces the expression of NKG2D ligands, leading to impaired NK-cell cytotoxicity and functionality. Article Snippet: .. The following primary antibodies were used for flow cytometry: anti-MICA (R&D, MAB1300), anti-MICB (R&D, MAB1599), antiULBP1 (R&D, MAB1380), anti-ULBP2 (R&D, MAB1298, with cross reactivity to ULBP5 and ULBP6), Article Title: Reovirus infection of tumor cells reduces the expression of NKG2D ligands, leading to impaired NK-cell cytotoxicity and functionality Article Snippet: .. The following primary antibodies were used for flow cytometry: anti-MICA (R&D, MAB1300), anti-MICB (R&D, MAB1599), anti-ULBP1 (R&D, MAB1380), anti-ULBP2 (R&D, MAB1298, with cross reactivity to ULBP5 and ULBP6), Expressing:Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer. Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), Staining:Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer. Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), Bioprocessing:Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer. Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), |
