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anti human ulbp3 antibodies  (R&D Systems)


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    R&D Systems anti human ulbp3 antibodies
    Anti Human Ulbp3 Antibodies, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ulbp3/Human+ULBP-3+Antibody/pm41529511-90-13-19
    Average 94 stars, based on 15 article reviews
    anti human ulbp3 antibodies - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
    Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), anti- ULBP1 (1:50; MAB1380, R&D Systems), anti-ULBP3 (1:50; MAB1517, R&D Systems), anti-ULBP4 (1:50; D ow nloaded from http://ahajournals.org by on January 12, 2024 MAB6285, R&D Systems), anti-NeuN (1:250; ab177487, abcam), anti-GFAP (1:1000; 13-0300, Invitrogen), anti- CX3CR1 (1:100; ab8021, abcam) and anti-CD68 (1:100; PA5-32330, Invitrogen). ..

    Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
    Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), antiULBP1 (1:50; MAB1380, R&D Systems), anti-ULBP3 (1:50; MAB1517, R&D Systems), anti-ULBP4 (1:50; MAB6285, R&D Systems), anti-NeuN (1:250; ab177487, abcam), anti-GFAP (1:1000; 13-0300, Invitrogen), antiCX3CR1 (1:100; ab8021, abcam) and anti-CD68 (1:100; PA5-32330, Invitrogen). ..

    Binding Assay:

    Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
    Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), anti- ULBP1 (1:50; MAB1380, R&D Systems), anti-ULBP3 (1:50; MAB1517, R&D Systems), anti-ULBP4 (1:50; D ow nloaded from http://ahajournals.org by on January 12, 2024 MAB6285, R&D Systems), anti-NeuN (1:250; ab177487, abcam), anti-GFAP (1:1000; 13-0300, Invitrogen), anti- CX3CR1 (1:100; ab8021, abcam) and anti-CD68 (1:100; PA5-32330, Invitrogen). ..

    Article Title: Impact of NKG2D Signaling on Natural Killer and T‐Cell Function in Cerebral Ischemia
    Article Snippet: .. Sections were heated in citrate buffer for antigen retrieval, rinsed in PBS and incubated for 1 hour in 5% BSA in PBS to prevent unspecific antibody binding prior to incubation with following primary antibodies: anti-CD3 (1:50; MCA1477, Bio-Rad), anti-CD4 (1:100; MAB3706, Millipore), anti-CD8 (1:50; MA1-80231, Thermo Scientific), anti-NKG2D (1:200; ab89807, abcam and 1:100; ab203353, abcam), anti-CD56 (1:200; RBK050, Zytomed), antiULBP1 (1:50; MAB1380, R&D Systems), anti-ULBP3 (1:50; MAB1517, R&D Systems), anti-ULBP4 (1:50; MAB6285, R&D Systems), anti-NeuN (1:250; ab177487, abcam), anti-GFAP (1:1000; 13-0300, Invitrogen), antiCX3CR1 (1:100; ab8021, abcam) and anti-CD68 (1:100; PA5-32330, Invitrogen). ..

    Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer.
    Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), anti-ULBP3 (clones 166,510; R&D Systems), anti-ULBP-2/5/6 (clones 165,903; R&D Systems) and anti-ULBP-4 (clones 709,116; R&D Systems). .. To measure the multiplicity of cell expansion, 2× cell suspension was mixed with Cell Proliferation Dye eFluorTM 670 (10 mM; eBioscience, Inc.; San Diego, CA, USA) in PBS at a ratio of 1:1 and incubated at 37°C for 10 min in the dark.

    Flow Cytometry:

    Article Title: Reovirus infection of tumor cells reduces the expression of NKG2D ligands, leading to impaired NK-cell cytotoxicity and functionality.
    Article Snippet: .. The following primary antibodies were used for flow cytometry: anti-MICA (R&D, MAB1300), anti-MICB (R&D, MAB1599), antiULBP1 (R&D, MAB1380), anti-ULBP2 (R&D, MAB1298, with cross reactivity to ULBP5 and ULBP6), anti-ULBP3 (R&D, MAB1517), anti-GLUT-1 (abcam, Ab150299), anti-IFNƔ (R&D, MAB285) , ant i -CD56 (BioLegend, 36503) , ant i -CD3 (ThermoFisher Scientific, 56-0038-82), anti-s1 (Sigma-Aldrich, MAB994-I-25UG), anti-CD4 (BioLegend, 344604), anti-CRT (Invitrogen, 902A), anti-ERp57 (BioLegend, 937301) and antiPVR (BioLegend, 337602). ..

    Article Title: Reovirus infection of tumor cells reduces the expression of NKG2D ligands, leading to impaired NK-cell cytotoxicity and functionality
    Article Snippet: .. The following primary antibodies were used for flow cytometry: anti-MICA (R&D, MAB1300), anti-MICB (R&D, MAB1599), anti-ULBP1 (R&D, MAB1380), anti-ULBP2 (R&D, MAB1298, with cross reactivity to ULBP5 and ULBP6), anti-ULBP3 (R&D, MAB1517), anti-GLUT-1 (abcam, Ab150299), anti-IFNƔ (R&D, MAB285), anti-CD56 (BioLegend, 36503), anti-CD3 (ThermoFisher Scientific, 56-0038-82), anti-σ1 (Sigma-Aldrich, MAB994-I-25UG), anti-CD4 (BioLegend, 344604), anti-CRT (Invitrogen, 902A), anti-ERp57 (BioLegend, 937301) and anti-PVR (BioLegend, 337602). ..

    Expressing:

    Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer.
    Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), anti-ULBP3 (clones 166,510; R&D Systems), anti-ULBP-2/5/6 (clones 165,903; R&D Systems) and anti-ULBP-4 (clones 709,116; R&D Systems). .. To measure the multiplicity of cell expansion, 2× cell suspension was mixed with Cell Proliferation Dye eFluorTM 670 (10 mM; eBioscience, Inc.; San Diego, CA, USA) in PBS at a ratio of 1:1 and incubated at 37°C for 10 min in the dark.

    Staining:

    Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer.
    Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), anti-ULBP3 (clones 166,510; R&D Systems), anti-ULBP-2/5/6 (clones 165,903; R&D Systems) and anti-ULBP-4 (clones 709,116; R&D Systems). .. To measure the multiplicity of cell expansion, 2× cell suspension was mixed with Cell Proliferation Dye eFluorTM 670 (10 mM; eBioscience, Inc.; San Diego, CA, USA) in PBS at a ratio of 1:1 and incubated at 37°C for 10 min in the dark.

    Bioprocessing:

    Article Title: Interleukin-15 and chemokine ligand 19 enhance cytotoxic effects of chimeric antigen receptor T cells using zebrafish xenograft model of gastric cancer.
    Article Snippet: Cells were incubated with corresponding flow cytometry antibodies (5 mL) at room temperature for 15 min, washed twice in phosphate-buffered saline (PBS) and run on a FACSCanto II flow cytometry system (BD Biosciences; San Jose, CA, USA) with an isotype control (BioLegend; San Diego, CA, USA) for each assay. .. To detect the expression of NKG2D ligand (NKG2DL) in AGS, HCG-27, and MKN45 cells, the cells were stained with NKG2D/CD314 Fc Chimera (clone Pro100Lys330; R&D Systems, Minneapolis, MN, USA) and specific monoclonal antibodies including anti-MHC class I chain-related protein A/ B (MICA/B; clone D7; R&D Systems), anti-UL16 binding protein 1 (ULBP-1; clone 170,818; R&D Systems), anti-ULBP3 (clones 166,510; R&D Systems), anti-ULBP-2/5/6 (clones 165,903; R&D Systems) and anti-ULBP-4 (clones 709,116; R&D Systems). .. To measure the multiplicity of cell expansion, 2× cell suspension was mixed with Cell Proliferation Dye eFluorTM 670 (10 mM; eBioscience, Inc.; San Diego, CA, USA) in PBS at a ratio of 1:1 and incubated at 37°C for 10 min in the dark.



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    Cytokine secretion, cytolytic activity and proliferation of CD19/NKG2DL tandem CAR T-cells co-cultured with CD19+ and CD19- Nalm-6 cells. (A) Expression of CD19 and NKG2DL in Nalm-6 and Nalm-6 CD19 KO cells. (B) Expression of individual NKG2DL using antibodies directed against MICA, MICB, ULBP1, <t>ULBP3</t> or ULBP2-5-6 (grey histograms) vs autofluorescence (white histograms). (C) Secretion of IFN-γ, TNF-α and IL-2 cytokines after a 24-hour co-culture at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells. (D) Cytolytic activity of CAR T-cells against Nalm-6 and Nalm-6 CD19 KO cells at 1:1 and 0.1:1 E:T ratio. Results are expressed as percentage of remaining cancer cells normalized to t0h timepoint. (E) Representative experiment showing CTV histograms of CAR T-cells after 4 days of co-culture without cancer cells or at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells (4 individual experiments were performed on 4 different donors). Adjusted P values (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Data presented as means (SD) of n=5. Each symbol denotes a different PBMC donor.
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    Image Search Results


    Cytokine secretion, cytolytic activity and proliferation of CD19/NKG2DL tandem CAR T-cells co-cultured with CD19+ and CD19- Nalm-6 cells. (A) Expression of CD19 and NKG2DL in Nalm-6 and Nalm-6 CD19 KO cells. (B) Expression of individual NKG2DL using antibodies directed against MICA, MICB, ULBP1, ULBP3 or ULBP2-5-6 (grey histograms) vs autofluorescence (white histograms). (C) Secretion of IFN-γ, TNF-α and IL-2 cytokines after a 24-hour co-culture at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells. (D) Cytolytic activity of CAR T-cells against Nalm-6 and Nalm-6 CD19 KO cells at 1:1 and 0.1:1 E:T ratio. Results are expressed as percentage of remaining cancer cells normalized to t0h timepoint. (E) Representative experiment showing CTV histograms of CAR T-cells after 4 days of co-culture without cancer cells or at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells (4 individual experiments were performed on 4 different donors). Adjusted P values (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Data presented as means (SD) of n=5. Each symbol denotes a different PBMC donor.

    Journal: Frontiers in Immunology

    Article Title: Tandem CAR T-cells targeting CD19 and NKG2DL can overcome CD19 antigen escape in B-ALL

    doi: 10.3389/fimmu.2025.1557405

    Figure Lengend Snippet: Cytokine secretion, cytolytic activity and proliferation of CD19/NKG2DL tandem CAR T-cells co-cultured with CD19+ and CD19- Nalm-6 cells. (A) Expression of CD19 and NKG2DL in Nalm-6 and Nalm-6 CD19 KO cells. (B) Expression of individual NKG2DL using antibodies directed against MICA, MICB, ULBP1, ULBP3 or ULBP2-5-6 (grey histograms) vs autofluorescence (white histograms). (C) Secretion of IFN-γ, TNF-α and IL-2 cytokines after a 24-hour co-culture at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells. (D) Cytolytic activity of CAR T-cells against Nalm-6 and Nalm-6 CD19 KO cells at 1:1 and 0.1:1 E:T ratio. Results are expressed as percentage of remaining cancer cells normalized to t0h timepoint. (E) Representative experiment showing CTV histograms of CAR T-cells after 4 days of co-culture without cancer cells or at 1:1 E:T ratio with Nalm-6 and Nalm-6 CD19 KO cells (4 individual experiments were performed on 4 different donors). Adjusted P values (*P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001) were determined by one-way ANOVA with Dunnet’s correction for multiple comparisons. Data presented as means (SD) of n=5. Each symbol denotes a different PBMC donor.

    Article Snippet: NKG2DL were detected using antibodies directed against human MICA (FAB1300P, R&D systems), MICB (FAB1599P, R&D systems), ULBP1 (FAB1380P, R&D systems), ULBP3 (FAB1517P, R&D systems), ULBP2-5-6 (FAB1298P, R&D systems) or using a recombinant human NKG2D (rhNKG2D-Fc, 1299-NK-050, R&D systems) detected with an anti-human IgG-Fc antibody (12-4998-82, ThermoFisher).

    Techniques: Activity Assay, Cell Culture, Expressing, Co-Culture Assay